CRCM

Helene Launay

helene.launayinserm.com
+33 (0)4.86.97.73.34

Current Project

Conformational diversity of intrinsically disordered proteins (IDPs) enables numerous protein-protein interactions that regulate the cell cycle. The molecular details on this conformational diversity is getting better understood, but how the conformational sampling regulates, or is regulated by, protein-protein interaction is of particular interest. I am combining experimental information on conformational sampling from both NMR and SAXS experiments to gain molecular details on protein-protein interactions involving IDPs.

Previous Work

Structural studies of the hepatitis C RNA polymerase NS5B in solution: protein-protein and protein-RNA interactions essential for viral RNA replication and conformational diversity of the RNA-polymerase.
UGSF-UMR 8576, Universite de Lille 1-CNRS

Co-translational folding of intact ribosome-bound nascent chain during protein synthesis.
ISMB, University College of London

Publications

8- H. Launay*, F. Blombach*, A. P. L. Snijders, V. Zorraquino-Salvo, H. Wu, B. de Koning, T. J.G. Ettema, A. Cavalli, M. Vendruscolo, M. J. Dickman, L. Cabrita, S. J.J. Brouns,A. La Teana, D. Benelli, P. Londei, J. Christodoulouand J. van der Oost (2014)Archaeal MBF1 binds to 30S and 70S ribosomes via itshelix-turn-helixdomain, Biochem. Journal, 461, BJ2013-1474. (* Co-Authorship)
7- H. Launay, B. Parent, A. Page, X. Hanoulle, G. Lippens, (2013) Dissociation kinetics of a binary complex in solution by protein displacement, AngewChemInt Ed Engl, 52(48) pp 12587-91. 
6- C. Smet-Nocca, H. Launay, J.-M. Wieruszeki, G. Lippens, I. Landrieu (2013) Unraveling a phosphorylation event in a folded protein by NMR spectroscopy: phosphorylation of the Pin1 WW domain by PKA, J. Biomol. NMR. 55(4) pp 323-337.
5- C.A. Waudby, H. Launay, L.D.Cabrita, J. Christodoulou (2013) Protein folding on the ribosome studied using NMR spectroscopy, ProgNuclMagnResonSpectrosc, 74 pp 57-75. 
4- C. Rosnoblet, B. Fritzinger, D. Legrand, H. Launay, J.-M. Wieruszeski, G. Lippens, X. Hanoulle (2012) Hepatitis C Virus NS5B and Host Cyclophilin A share a common binding site on NS5A, J. Biol. Chem. 287(53) pp 44249-60. 
3- F. Blombach, H. Launay, V. Zorraquino, D. C. Swarts, L. D. Cabrita, D. Benelli, J. Christodoulou, P. Londei, J. van der Oost (2011) An Hflx-type GTPase from Sulfolobussolfataricus binds to the 50S ribosomal subunits in all nucleotide-bound states, J. Bacteriol, 193(11) pp 2861. 
2- L. D. Cabrita, S.-T. D. Hsu, H. Launay, C. M. Dobson, J. Christodoulou (2009) Probing ribosome-bound nascent chain complexes produced in vivo by NMR spectroscopy, PNAS, 106(11) pp 22239. 
1- S.-T. D. Hsu, P. Fucini, L. D. Cabrita, H. Launay, C. M. Dobson, J. Christodoulou (2007) Structure and dynamics of a ribosome-bound nascent chain by NMR spectroscopy, PNAS, 104(42) pp 16516.